Author by: Ashok Kumar Language: en Publisher by: Discovery Publishing House Format Available: PDF, ePub, Mobi Total Read: 63 Total Download: 567 File Size: 52,6 Mb Description: The present title Protein Biotechnology tries to capture the field of biotechnology in its whole some entirety in a manner that is concise and easily understood by the students. Enzymatic proteins play a vital role in degradation and fermentation providing a well known example but more recent developments of an over increasing range of raw materials have led to a wider and greater impact on the biotechnology industry.

• Difference Between Biotechnology And Biochemistry
• Biochemistry

Because it is undergoing such rapid growth, there will almost certainly be many examples of protein biotechnology in actual application when this book appears which had not reached that stage when it was written. For this reason we have allowed ourselves to include some examples which look as if they are likely to find application in the near future, despite our endeavour to illustrate the technology be examples that are actually in use, rather than possible processes that might one day find application but all too often really illustrate unfulfilled promise.

Pharmaceutical Biotechnology offers students taking Pharmacy and related Medical and. Dr Gary Walsh. Proteins: Biochemistry and Biotechnology. Proteins Biochemistry and Biotechnology 2e. Gary Walsh: Isbn: 860. Provides a comprehensive overview of all aspects of protein biochemisty and protein.

Author by: Felix Franks Language: en Publisher by: Springer Science & Business Media Format Available: PDF, ePub, Mobi Total Read: 24 Total Download: 528 File Size: 46,5 Mb Description: Proteins are the servants of life. They occur in all com- nent parts of living organisms and are staggering in their fu- tional variety, despite their chemical similarity.

Even the simplest single-cell organism contains a thousand different p- teins, fulfilling a wide range of life-supporting roles. Additions to the total number of known proteins are being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation. The total international protein literature could fill a medi- sized building and is growing at an ever-increasing rate.

The reader might be forgiven for asking whether yet another book on proteins, their properties, and functions can serve a useful purpose. An explanation of the origin of this book may serve as justification.

The authors form the tutorial team for an int- sive postexperience course on protein characterization or- nized by the Center for Professional Advancement, East Brunswick, New Jersey, an educational foundation. The course was first mounted in Amsterdam in 1982 and has since been repeated several times, in both Amsterdam and the US, with participants from North America and most European countries. In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course. Today, isolated proteins for food use are extracted from yeasts, fungal sources, legumes, oilseeds, cereals, and leaves. Author by: Gary Walsh Language: en Publisher by: John Wiley & Sons Format Available: PDF, ePub, Mobi Total Read: 35 Total Download: 143 File Size: 53,7 Mb Description: Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine.

Fully updated throughout with new or fundamentally revised sections on proteomics as, bioinformatics, protein glycosylation and engineering, well as sections detailing advances in upstream processing and newer protein applications such as enzyme-based biofuel production this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies. This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology. Includes numerous case studies.

Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology. Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production 'With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. ' (Engineering in Life Sciences, 2004; Vol 5; No. 5) “Few texts would be considered competitors, and none compare favorably.'

(Biochemistry and Molecular Education, July/August 2002) '.The book is well written, making it informative and easy to read.' (The Biochemist, June 2002). Author by: Vijai K.

Pasupuleti Language: en Publisher by: Springer Science & Business Media Format Available: PDF, ePub, Mobi Total Read: 20 Total Download: 599 File Size: 49,9 Mb Description: Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following.

Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates.

This is extremely important when capacities are limited. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers. Author by: Lilia Alberghina Language: en Publisher by: CRC Press Format Available: PDF, ePub, Mobi Total Read: 59 Total Download: 693 File Size: 42,7 Mb Description: Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered.

It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering. The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery. International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.

Author by: Roger L. Lundblad Language: en Publisher by: CRC Press Format Available: PDF, ePub, Mobi Total Read: 18 Total Download: 155 File Size: 52,8 Mb Description: Reflecting the versatility of the author’s science and the depth of his experience, Application of Solution Protein Chemistry to Biotechnology explores key contributions that protein scientists can make in the development of products that are both important and commercially viable, and provides them with tools and information required for successful participation. One of the of the world’s most respected protein researchers, Roger Lundblad does not succumb to the notion that new is always better. The application of protein science to the practice of commercial biotechnology is traced to the underlying basic solution protein chemistry.

It is only by achieving this understanding that the full potential of protein science may be obtained in the development and characterization of the diverse products of modern biotechnology. Lundblad also goes far beyond the biopharmaceutical applications that are often equated with protein science today to demonstrate the field’s unique versatility. From the making of bread and the invention of adhesives to the production of pharmaceuticals and the development of recombinant DNA products— in each of these products, the role of the protein chemist remains prominent.

The important point is that classical protein chemistry is a critical part of the practice of biotechnology in the marketplace.

. Aland Islands.

Albania. Andorra. Armenia. Austria.

Azerbaijan. Belarus. Belgium.

Bosnia and Herzegovina. Bulgaria. Croatia. Cyprus. Czech Republic. Denmark.

Estonia. Finland. France. Georgia.

Germany. Gibraltar. Greece. Greenland. Holy See (Vatican City State).

Hungary. Iceland. Ireland. Italy. Latvia.

Liechtenstein. Lithuania.

Luxembourg. Macedonia.

Malta. Moldova. Monaco. Montenegro. Netherlands. Norway.

Difference Between Biotechnology And Biochemistry

Poland. Portugal. Romania. Russia.

Serbia. Slovakia. Slovenia.

Spain. Sweden. Switzerland. Turkey.

Ukraine. United Kingdom. American Samoa. Australia. Bangladesh.

Bhutan. British Indian Ocean Territory. Brunei. Cambodia. China. Christmas Island.

Cocos (Keeling) Islands. Cook Islands. Fiji.

Guam. India. Indonesia. Japan. Kazakhstan.

Korea (the Republic of). Kyrgyzstan.

Laos. Malaysia. Maldives. Mongolia. Myanmar. Nepal.

New Zealand. Pakistan. Papua New Guinea.

Philippines. Samoa. Singapore.

Solomon Islands. Sri Lanka. Tajikistan. Thailand. Timor-Leste. Tonga. Turkmenistan.

Uzbekistan. Vanuatu. Vietnam. Description Pharmaceutical Biotechnology offers students taking Pharmacy and related Medical and Pharmaceutical courses a comprehensive introduction to the fast-moving area of biopharmaceuticals.

With a particular focus on the subject taken from a pharmaceutical perspective, initial chapters offer a broad introduction to protein science and recombinant DNA technology- key areas that underpin the whole subject. Subsequent chapters focus upon the development, production and analysis of these substances. Finally the book moves on to explore the science, biotechnology and medical applications of specific biotech products categories. These include not only protein-based substances but also nucleic acid and cell-based products. introduces essential principles underlining modern biotechnology- recombinant DNA technology and protein science. an invaluable introduction to this fast-moving subject aimed specifically at pharmacy and medical students.

includes specific ‘product category chapters’ focusing on the pharmaceutical, medical and therapeutic properties of numerous biopharmaceutical products. entire chapter devoted to the principles of genetic engineering and how these drugs are developed. includes numerous relevant case studies to enhance student understanding. no prior knowledge of protein structure is assumed. 1 Pharmaceuticals, biologics and biopharmaceuticals. 1.1 Introduction to pharmaceutical products.

1.2 Biopharmaceuticals and pharmaceutical biotechnology. 1.3 History of the pharmaceutical industry. 1.4 The age of biopharmaceuticals. 1.5 Biopharmaceuticals: current status and future prospects. 2 Protein structure. 2.1 Introduction.

Biochemistry

2.2 Overview of protein structure. 2.3 Higher level structure.

2.4 Protein stability and folding. 2.5 Protein post-translational modifi cation.

3 Gene manipulation and recombinant DNA technology. 3.1 Introduction. 3.2 Nucleic acids: function and structure. 3.3 Recombinant production of therapeutic proteins.

3.4 Classical gene cloning and identifi cation. 4 The drug development process. 4.1 Introduction. 4.2 Discovery of biopharmaceuticals. 4.3 The impact of genomics and related technologies upon drug discovery. 4.4 Gene chips.

4.5 Proteomics. 4.6 Structural genomics. 4.7 Pharmacogenetics.

4.8 Initial product characterization. 4.9 Patenting. 4.10 Delivery of biopharmaceuticals. 4.10.3 Nasal, transmucosal and transdermal delivery systems. 4.11 Preclinical studies. 4.12 Pharmacokinetics and pharmacodynamics.

4.13 Toxicity studies. 4.14 The role and remit of regulatory authorities. 4.15 Conclusion.

5 Sources and upstream processing. 5.1 Introduction. 5.2 Sources of biopharmaceuticals. 5.3 Upstream processing. 6 Downstream processing. 6.1 Introduction.

6.2 Initial product recovery. 6.3 Cell disruption. 6.4 Removal of nucleic acid.

6.5 Initial product concentration. 6.6 Chromatographic purifi cation. 6.7 High-performance liquid chromatography of proteins.

6.8 Purifi cation of recombinant proteins. 6.9 Final product formulation. 7 Product analysis. 7.1 Introduction.

7.2 Protein-based contaminants. 7.3 Removal of altered forms of the protein of interest from the product stream. 7.4 Detection of protein-based product impurities. 7.5 Immunological approaches to detection of contaminants. 7.6 Endotoxin and other pyrogenic contaminants. 8 The cytokines: The interferon family. 8.1 Cytokines.

8.1.1 Cytokine receptors. 8.1.2 Cytokines as biopharmaceuticals. 8.2 The interferons. 8.3 Interferon biotechnology. 8.4 Conclusion. 9 Cytokines: Interleukins and tumour necrosis factor.

9.1 Introduction. 9.2 Interleukin-2. 9.3 Interleukin-1. 9.4 Interleukin-11.

9.5 Tumour necrosis factors. 10 Growth factors. 10.1 Introduction. 10.2 Haematopoietic growth factors. 10.3 Growth factors and wound healing.

11 Therapeutic hormones. 11.1 Introduction.

11.2 Insulin. 11.3 Glucagon. 11.4 Human growth hormone. 11.5 The gonadotrophins. 11.6 Medical and veterinary applications of gonadotrophins. 11.7 Additional recombinant hormones now approved. 11.8 Conclusion.

12 Recombinant blood products and therapeutic enzymes. 12.1 Introduction. 12.2 Haemostasis. 12.3 Anticoagulants. 12.4 Thrombolytic agents. 12.5 Enzymes of therapeutic value.

13 Antibodies, vaccines and adjuvants. 13.1 Introduction. 13.2 Traditional polyclonal antibody preparations. 13.3 Monoclonal antibodies. 13.4 Vaccine technology. 13.5 Adjuvant technology. 14 Nucleic-acid- and cell-based therapeutics.

14.1 Introduction. 14.2 Gene therapy.

14.3 Vectors used in gene therapy. 14.4 Gene therapy and genetic disease. 14.5 Gene therapy and cancer. 14.6 Gene therapy and AIDS. 14.7 Antisense technology. 14.8 Oligonucleotide pharmacokinetics and delivery. 14.9 Aptamers.

14.10 Cell- and tissue-based therapies. 14.11 Conclusion.